`install.packages("BiBitR")`

```
install.packages("devtools") # If not yet installed on your R Version
::install_github("hadley/devtools") # Only run this if your currently installed
devtools# devtools version is <= 1.12 (recursive dependencies bug)
::install_github("ewouddt/BiBitR") devtools
```

Should the installation of `BiBitR`

or
`devtools::install_github("hadley/devtools")`

throw an error,
please install the dependencies manually, then try:

```
install.packages(c("flexclust","biclust"))
::install_github("ewouddt/BiBitR") devtools
```

`BiBitR`

is a simple R wrapper which directly calls the
original Java code for applying the BiBit algorithm after which the
output is transformed to a `Biclust`

S4 class object. The
original Java code can be found at http://eps.upo.es/bigs/BiBit.html by Domingo S.
Rodriguez-Baena, Antonia J. Perez-Pulido and Jesus S. Aguilar-Ruiz.

More details about the **BiBit** algorithm can be found
in:

The `bibit`

function uses the original Java code directly
(with the intended input and output). Because the Java code was not
refactored, the `rJava`

package could not be used.

The `bibit`

function does the following:

- Convert R matrix to a
`.arff`

output file. - Use the
`.arff`

file as input for the Java code which is called by`system()`

. - The outputted
`.txt`

file from the Java BiBit algorithm is read in and transformed to a`Biclust`

object.

Because of this procedure, there is a chance of *overhead*
when applying the algorithm on large datasets. Make sure your machine
has enough RAM available when applying to big data.

**Note:** If you want to circumvent the internal R
function to convert the matrix to `.arff`

format, look at the
documentation of the `arff_row_col`

parameter of the
`bibit`

function. You can input the original input files for
the java algorithm with this parameter. The original input files can
also be generated with the `make_arff_row_col`

function in
the package.

The BiBit algorithm was also slightly adapted to allow some noise in
the biclusters. This can be done with the `bibit2`

function
in the `BiBitR`

package. It is the same function as
`bibit`

, but with an additional new noise parameter which
allows 0’s in the discovered biclusters.

`bibit2`

follows the same steps as described in the
Details section above. Following the general steps of the BiBit
algorithm, the allowance for noise in the biclusters is inserted in the
original algorithm as such:

- Binary data is encoded in bit words.
- Take a pair of rows as your starting point.
- Find the maximal overlap of 1’s between these two rows and save this as a pattern/motif. You now have a bicluster of 2 rows and N columns in which N is the number of 1’s in the motif.
- Check all remaining rows if they match this motif,
*however*allow a specific amount of 0’s in this matching as defined by the`noise`

parameter. Those rows that match completely or those within the allowed noise range are added to bicluster. - Go back to
*Step 2*and repeat for all possible row pairs.

**Note:** Biclusters are only saved if they satisfy the
`minr`

and `minc`

parameter settings and if the
bicluster is not already contained completely within another
bicluster.

What you will end up with are biclusters not only consisting out of 1’s, but biclusters in which 2 rows (the starting pair) are all 1’s and in which the other rows could contain 0’s (= noise).

**Note:** Because of the extra checks involved in the
noise allowance, using noise might increase the computation time a
little bit.

The `noise`

parameter determines the amount of zero’s
allowed in the bicluster (i.e. in the extra added rows to the starting
row pair) and can take on the following values:

`noise=0`

: No noise allowed. This gives the same result as using the`bibit`

function.`0<noise<1`

: The noise parameter will be a noise percentage. The number of allowed 0’s in a (extra) row in the bicluster will depend on the column size of the bicluster. More specifically`zeros_allowed = ceiling(noise * columnsize)`

. For example for`noise=0.10`

and a bicluster column size of`5`

, the number of allowed 0’s would be`1`

.`noise>=1`

: The noise parameter will be the number of allowed 0’s in a (extra) row in the bicluster independent from the column size of the bicluster. In this noise option, the noise parameter should be an integer.

Normally you would apply BiBit (with/without noise) directly on the binary data. However due to the nature of the algorithm, namely starting an exhaustive search from each row-pair, it is also possible to look for specific patterns of interest.

To do this, simply add 2 (artificial) identical rows which contain the pattern/motif of interest (e.g. 2 rows with 1’s in specific columns and 0 everywhere else). You can do this multiple times if multiple patterns/motifs are of interest.

This procedure is currently implemented in the package in the
`bibit3`

function. It allows to drive the BiBit algorithm to
only look for one or multiple full or sub patterns (which increases the
speed). See the Documentation of `bibit3`

for more info.

The package also allows you extend the bibit biclusters in the column
dimension. More info can be found in the documentation of the
`bibit2`

, `bibit3`

and
`bibit_columnextension`

functions. The goal of the procedure
is:

*BiBit with Noise Allowance*: Try to add noise to the 2 initial perfect rows.*Bibit With Patterns (Alternative Strategy)*: Do the rows, which overlap partly or fully with the given pattern, have other similarities outside the given pattern?

Looking for Noisy Biclusters in large data using BiBit
(`bibit2`

) often results in many (overlapping) biclusters. In
order decrease the number of biclusters and find larger meaningful
patterns which make up noisy biclusters, the following workflow
(`BiBitWorkflow`

) can be applied. Note that this workflow is
primarily used for data where there are many more rows (e.g. patients)
than columns (e.g. symptoms). For example the workflow would discover
larger meaningful symptom patterns which, conditioned on the allowed
noise/zeros, subsets of the patients share.

A Biclust S4 Class object.

```
library(BiBitR)
<- matrix(sample(c(0,1),100*100,replace=TRUE,prob=c(0.9,0.1)),nrow=100,ncol=100)
data 1:10,1:10] <- 1 # BC1
data[11:20,11:20] <- 1 # BC2
data[21:30,21:30] <- 1 # BC3
data[<- data[sample(1:nrow(data),nrow(data)),sample(1:ncol(data),ncol(data))]
data
<- bibit(data,minr=2,minc=2)
result1
result1MaxBC(result1)
<- bibit2(data,minr=5,minc=5,noise=0.2)
result2
result2MaxBC(result2)
<- bibit2(data,minr=5,minc=5,noise=3)
result3
result3MaxBC(result3)
```